The bout will be decided in Spain after the first match ended in a one-one draw, as Bayern answered an early goal and resisted after a late red card.
Match Replays
Lyonnes need to rally to punch their ticket to the final, as Arsenal secured a comeback win at home thanks to two second-half goals in the first leg.
Arsenal eliminated OH Leuven and Chelsea in the previous two rounds, while Lyoness were dominant in the group stage before knocking out Wolfsburg.
Bayern survived a rollercoaster against Man. United in the quarter-finals. Instead, Barcelona ousted Paris FC and Real Madrid rather easily.
Wolfsburg will attempt to defend their small advantage, as they scored early on their turf and held off Lyonnes for the remainder of the game.
Barcelona have more than one foot in the semifinals, as they blew out Real Madrid on the road, piling on six goals and conceding twice last week.
Chelsea will try to stage a comeback in front of their fans after Arsenal prevailed 3-1 in the first leg of the London derby in the Round of Eight.
Bayern outlasted Man. United at home, emerging victorious with a 3-2 result, but the qualification remains wide-open heading into the return match.
Man. United prevailed handily in both matches versus Atlético Madrid in the past round. Bayern have had just one blemish in the competition so far.
Real Madrid and Barcelona renew their historic rivalry. The latter have been dominant so far, while the hosts edged out Paris FC in the playoffs.
Arsenal scored seven goals in the two legs versus OH Leuven. Chelsea are entering the knockout stage after closing the previous one in third place.
Wolfsburg eliminated Juventus in the playoffs thanks to a strong showing at home. Lyonnes are undefeated and finished second in the league phase.